Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations
نویسندگان
چکیده
منابع مشابه
Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris.
Protein expression in the microbial eukaryotic host Pichia pastoris offers the possibility to generate high amounts of recombinant protein in a fast and easy to use expression system. As a single-celled microorganism P. pastoris is easy to manipulate and grows rapidly on inexpensive media at high cell densities. Being a eukaryote, P. pastoris is able to perform many of the post-translational mo...
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A recombinant lactase was expressed in Pichia pastoris, resulting in enzymatic activity of 3600 U/mL in a 5 L fermenter. The lactase product was subjected to a series of toxicological tests to determine its safety for use as an enzyme preparation in the dairy industry. This recombinant lactase had the highest activity of all recombinant strains reported thus far. Acute oral toxicity, mutagenici...
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LEVEL: INTERMEDIATE Ever since Invitrogen of Carlsbad, CA (www.invitrogen.com) acquired the distribution rights in 1993 for the Pichia pastoris yeast protein expression system, P. pastoris has gained widespread popularity, as witnessed by an increasing number of publications (Figure 1). The expression system was originally developed as a single-cell protein production platform by Philips Petrol...
متن کاملIsotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris.
The methylotrophic yeast Pichia pastoris is now an established expression system for the production of recombinant protein for nuclear magnetic resonance (NMR) studies. It is capable of expressing high levels of heterologous proteins and possesses the ability to perform many of the posttranslational modifications of higher eukaryotes. Here, we describe efficient methods for the production of un...
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Pontryagin's Maximum Principle has been applied for optimization of secreted proteins from Pichia pastoris fed-batch fermentation. The objective of this work is to maximize the total accumulated product per unit operation time under different given conditions and system constraints. To obtain optimal solutions, an automated curve-fitting software, Table Curve 2D, was employed to construct the n...
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ژورنال
عنوان ژورنال: Current Protocols in Protein Science
سال: 2020
ISSN: 1934-3655,1934-3663
DOI: 10.1002/cpps.104